Acta Medicinæ Legalis Vol. XLIV 1994: XVIth Congress of the by A. Busuttil (auth.), Professor Dr. Patrice Mangin, Dr.

By A. Busuttil (auth.), Professor Dr. Patrice Mangin, Dr. Bertrand Ludes (eds.)

This publication includes the medical studies of contemporary advances in criminal medication and at once comparable sciences.
Various themes are coated by means of prime scientists within the fields of medical felony medication, physically harm overview, and forensic odontoanthropology, and likewise organic advances comparable to toxicology and DNA know-how together with polymorphic DNA platforms resembling VNTR, STR, and different PCR dependent polymorphysms. moral difficulties and forensic psychiatry and jurismedicine also are treated.
Thus, this quantity bargains priceless information and strategies that may be at once utilized by scientists operating within the fields of forensic and felony drugs, forensic technological know-how, toxicology, inhabitants genetics, anthropology, forensic odontology, ethics, and forensic psychiatry.

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Extra resources for Acta Medicinæ Legalis Vol. XLIV 1994: XVIth Congress of the International Academy of Legal Medicine and Social Medicine, Strasbourg, France, 31 May – 2 June, 1994 / XVIème Congrès de l’Acadèmie Internationale de Mèdecine Lègale et de Mèdecine Sociale Stra

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1) for the entire procedure. 15 (data not shown) identical fingerprinting patterns could be generated with both methods. 27 Furthermore, in fifty cases with established mother-child-father trios we have not seen any significant difference between the manual and the automated DNA extraction method. Conclusion: The Autogen 540 DNA extractor is a suitable instrument for isolating DNA from whole blood samples in an automated manner. The quality and the yield of the resulting DNA are identical to that obtained by manual methods.

In most cases further purifications are not required, however the specimens to be investigated will determine the best startegy to be followed. References 1- Hocbmeister, M. ; Borer, U; Eggman, U; Corney, C. and Dirnhofer, R (1991). Typing of DNA extracted from compact bone from human remains. Journal of Forensic Science 36: 1649-1661. ; Baer, D. B. and Budowle, B. (1991). Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQalpha gene. Int. J. of Legal Medicine 104: 145148.

4 22,~ FT1 (chorionic ... <4 18,2<4. ) rnoIhIr'. 3. 13. Ie. -tIgMion to dlecard .... me. ot. ,.. J!! '! '! '! ubl~O ~E2nna Concluding remarks The PCR-amplification of six sequence polymorphisms (HLA-DQA1, LDLR. GYPA, HBGG. 0758 and GC) and three STRs (HUMTH01. HUMFABP and HUMVWA) using as a template of amplification the DNA extracted by phenol-chloroform I Centricon-100 from eight formalin-fixed, paraffin-embedded tissue blocks has worked very effiCiently in all cases. In contrast, no amplifiation products were generated at locus 01580, but one case.

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