Advances in Enzymology and Related Areas of Molecular by Alton Meister

By Alton Meister

Protein Tyrosine Phosphatases: Mechanism of Catalysis and Substrate Specificity (Z.-Y.

Zhang & J. Dixon).

Heterologous Expression of Mammalian P450 Enzymes (M. Waterman).

Aspartate Transcarbamylase from Escherichia Coli: job and legislation (W. Lipscomb).

constitution and Mechanism of Bovine Lens Leucine Aminopeptidase (H. Kim & W. Lipscomb).


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Proc. N o t / . Accrd, Sci, USA, 86, 8698-8702 (198’9). 43. Durnphy, W. C. and Kurnagai, A , , CelI, 67, 189-196 (1991). 44. Gautier, J . , Solomon, M. , Booher, R. , Bazan, J . F.. and Kirschner. M. , Cell, 67. 197-211 (1991). PROTEIN TYROSINE PHOSPHATASES 33 45. Millar. J . , McGowan, C. , Jones. , 10, 4302-4309 (1991). 46. Pot. D. A , , Woodford, T. , Haun, R. , and Dixon, J. I. B i d . , 266, 19688-19696 (1991). 47. Guan. K. L.. Huan, R. , Watson. S. , Geahlen. R. L.. and Dixon, J. E.. 01..

ZHONG-YIN ZHANG AND JACK E. DIXON Birge, R. , Fajardo, J. , Chou, M. , and Cantley, L. , Cell, 72, 767-778 (1993). , Shoelson, S. , Cell. 72, 779-790 (1993). , Science, 259, 1607-161 I (1993). Vogel, W . , Science, 259, 1611-1614 (1993). Chernoff, J . , Schievella, A. , Jost, C. , Erikson, R. L.. and Neel. B. , Proc. Nutl. Acad. Sci. USA, 87, 2735-2739 (1%). Brown-Shimer, S. ,Johnson, K. , Lawrence, J. , Bruskin. A.. Green, N . , and Hill, D. , Proc. Nutl. A w d . Sci. U S A , 87, 51484152 (1990).

Because COS cells contain the protein synthetic machinery and subcellular compartments of animal cells, they have proven to be very good hosts for the study of recombinant P450. On the negative side, the COS system involves transient transfection, and the level of expression is dependent on the efficiency of transfection. Transfection efficiency can be as low as 10% and it must be assumed that, although COS cells are useful for an investigation of enzymatic properties of recombinant P450s, they are not suitable for production of sufficient quantities of these enzymes for biophysical studies.

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